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Image Search Results
Journal: Nature Communications
Article Title: Intracellular delivery of protein drugs with an autonomously lysing bacterial system reduces tumor growth and metastases
doi: 10.1038/s41467-021-26367-9
Figure Lengend Snippet: a ID Salmonella delivery of NIPP1-CD caused more death ( red , white arrows ) in Hepa 1-6 cells compared to controls ( P = 0.0021, n = 6). b In microfluidic tumor masses, delivery of NIPP1-CD caused more cell death ( red ) than bacterial controls. The percentage of dead cells increased with time as Salmonella invaded into cells and delivered protein ( P = 0.042, 0.017, 0.014, 0.017, 0.024, 0.030, and 0.039 at 6.5, 7, 7.5, 8, 8.5, 9, and 9.5 h, respectively, n = 4). c For multiple ( n = 4) tumor masses, as shown in b , NIPP1-CD significantly increased cell death ( P = 0.0177). d NIPP1-CD ID Salmonella was intravenously administered to BALB/c mice with subcutaneous 4T1 tumors. After 31 days, Salmonella ( black arrow , green ) and delivered NIPP1-CD ( white arrows , red ) were dispersed throughout the tissue. Data are shown as means ± SEM. Statistical comparisons in a – c are two-tailed, unpaired Student’s t -tests with asterisks indicating significance (* P < 0.05; ** P < 0.01). Images in a, b , and d are representative of 6, 4, and 3 independent biological samples, respectively. Scale bars in a and b are 100 µm, and in d the scale bar is 10 µm.
Article Snippet: After 31 days, tumors were excised and stained for Salmonella ( Abcam , catalog # ab69253; 1:100 dilution) and
Techniques: Two Tailed Test